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KMID : 1004820120130010083
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Journal of Biomedical Research
2012 Volume.13 No. 1 p.83 ~ p.92
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Evaluation of Hydroxyl Radical Reduction Activity of Red Ginseng Extract Using ESR Spectroscopy
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Han Jin-Yi
Hong Jin-Tae
Nam Sang-Yoon
Oh Ki-Wan
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Abstract
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This study was conducted in order to investigate the reduction activity of red ginseng extract (RGE; Panax ginseng, C. A. Meyer) on hydroxyl radical (?OH) using an electron spin resonance (ESR) spectrometer and spin-trapping techniques. ?OH generated by a Fenton Reaction System was trapped by 5, 5-dimethyl-l-pyrroline-N oxide (DMPO). The decay rate showed approximately pseudo-first order kinetics over the period of measurement (by 10 min), and the half lifetime of the DMPO/?OH signal was estimated as approximately 8.15 min. However, the half lifetime of RGE/?OH was estimated as approximately 7.5 min, and the half lifetime of RGE was higher than that of DMPO/?OH adduct only. The order of reduction activities was ascorbic acid > N, N?-dimethylthiourea (DMTU) > RGE > trolox > mannitol in the Fenton Reaction System. Thus, these observations indicate that RGE reaction with ?OH has relative reduction activity. The second-order rate constant of RGE/?OH may be 3.5~4.5 × 109 M-1 ? S-1.
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KEYWORD
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Electron Spin Resonance (ESR), Reduction activity, Hydroxyl radical (?OH), Spin-trapping, Red Ginseng Extract (RGE)
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